Yes, you can migrate Exchange Server to Office 365 with minimal downtime by planning the migration in batches and performing the final cutover during non-business hours. While Microsoft offers native migration methods, they can be complex for larger environments.

For a simpler approach, SysTools Exchange to Office 365 Migrator helps migrate Exchange mailboxes directly to Microsoft 365 while preserving emails, contacts, calendars, folder hierarchy, and metadata. It supports bulk and incremental migration, making the process smoother with minimal disruption.